HPLC Columns and Cartridges

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By david jones Posted on Jan 10, 2024
In Category - Health & Fitness

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James Anderson
Abstract

High-performance liquid chromatography (HPLC) columns are used for the analysis or preparation of separated compounds from complex mixtures. The pressurized liquid solvent containing the sample mixture passes through a column filled with a solid adsorbent material. Since each component in the sample interacts with the adsorbent material slightly differently, the components with different flow rates are separated as they flow out of the column. According to separation mechanism, common HPLC column types are classified as normal/reversed phase, mixed-mode, ion exchange, ligand exchange, and affinity column formats.

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